CSR&TI, BERHAMPORE, WEST BENGAL
HIGHLIGHTS OF CONCLUDED RESEARCH PROJECTS WITH REFERENCE TO ACHIEVEMENT OF THE OBJECTIVES, OUTPUT AND UTILITY

Sl. No.

Project Code, Title & period

Objectives

Out-put of the project

Utility of the out-put

Impact on Silk Industry

DURING THE YEAR:  2015-2016

1

PIB-3424: Development of low temperature stress tolerant mulberry genotypes for sub- tropical plains. (Jan., 09 – Dec., 15)

Development of low temperature stress tolerant mulberry genotype capable of providing higher leaf yield during Agrahayani (Nov.) and Falguni (Feb.) silkworm rearing seasons than the ruling variety, S-1635.

  • Seven new genotypes were identified based on sprouting duration and electrical conductivity.
  • Annual leaf yield/ plant (kg) in seven genotypes were  C-45  (1.94 kg),  C- 108 (2.12 kg), C-212 (1.97 kg), C-225 (1.87 kg), C-232 (1.85 kg), C-371 (1.74 kg) and C-384 (2.51 kg) while 1.71 kg was in S 1635 (check).

Further evaluation of selected seven new genotypes i.e., C-29, C-33, C-45, C-108, C-212, C-225
and C-384 under Final Yield Trial and Mulberry Variety Authorization trial.

High   yielding  and qualitatively superior mulberry variety for
increasing mulberry & cocoon production thereby economic development to the farmers.

2

PPS-3452: Terrestrial Carbon sequestration for sustained high productivity of quality mulberry. (July.,11 to June, 15)

To enumerate the enhanced organic carbon stock of the soil due to the induction of altered farming practices in mulberry dim fit to carbon sequestration with comparison to existing one.

Among six farming practices, mulberry growing under moderate tillage with grass cover registered the highest leaf productivity of 38.72 ton ha-1 year-1, the highest CSP of 6.90 ton ha-1 year-1 and the highest SOCS of 40.16 mg ha-1 against the existing farming practice (intensive tillage) leaf productivity 38.16 ton, CSP 6.53 ton and SOCS 35.25 mg ha-1, respectively.

A program  entitled “Testing of carbon capturing efficiency of mulberry in different locations” has already been initiated at RSRSs level.

To increase mulberry leaf
productivity, Soil organic carbon stock  (SOCS)  and Carbon Sequestration Potential (CSP).

3

APS-3497: Studies on the environmental effect on P1 rearing, its’ grainage performance followed by commercial rearing of Silkworm Bombyx mori L., during unfavourable seasons of West Bengal. (May, 13 to Apr., 15).

Determination of the effect of environmental factors on P1 seed crop rearing during adverse crop seasons at farmers’ level and its subsequent effects on commercial grainage performance and finally on commercial rearing at farmers’ level.

Environmental factors such as, temperature and relative humidity influence on silkworm seed  crop rearing, reproductive potential and on subsequent commercial grainage performance.

Solving the grainage problems and to overcome the stress during unfavourable crop seasons in West Bengal

Information would be useful in increasing silkworm layings production.

4

AIB-3496: Development of high temperature and high humidity tolerant bivoltine breeds of silkworm (Bombyx mori L.).
(Jul., 12 to Jun., 15)

  • Determination                               of                             LD50                               in silkworm
  • To develop a method of induction for thermal stress in silkworm.
  • Development of high temperature and high humidity tolerant breed.

Screening of silkworm breeds under high temperature (35±1°C) and high humidity (85±5%) conditions, 10 breeds were short listed as breeding resource materials based on overall performance with special emphasis on pupation rate. Based on overall performance at farmers’ field, M6DPC x (SK6 x SK7) (Multi x Bi. hybrid) in West Bengal and Jharkand states and bivoltine hybrid, B.Con.1 x B.Con.4 in West Bengal, Jharkand and North Eastern states was recommended to rear during favourable seasons (Agrahayani, Falguni and Baishaki).

Selected 10 breeds with higher pupation at high temperature and high humidity conditions, will be utilised for further breeding work.

Hardy Multi x bivoltine and bivoltine x bivoltine hybrids for increasing cocoon and silk production.

5

AIB-3531: Authorization trials of silkworm hybrids in Eastern and North Eastern India. (Aug., 14 to July, 15)

  • To evaluate the newly developed, Multi x Bi and Bi x Bi hybrids at the farmers level in Eastern & North Eastern Zone
  • To popularize the newly developed hybrids at the farmers level in Eastern & North Eastern Zone
  • To analyze the  data of the tested hybrids in Eastern & North Eastern Zone.
  • To identify the suitable hybrids for the Eastern & North Eastern Zone.
  • Recommendation of the hybrids for the Eastern and North Eastern zone for commercial exploitation.
  • Multi x Bi hybrid [M6DPC x (SK6 x SK7)]) performed cocoon  yield  50.4  kg  against

46.9 kg/ 100 dfls of Nistari x (SK6 x SK7).

  • Bivoltine foundation cross B.Con.1 x B.Con.4 performed cocoon   yield   51.6kg   against

47.6 kg/100 dfls of (SK6 x SK7) during Agrahayani/Autumn, Falguni/ Spring and Biashaki crop seasons.

New hybrids will be authorized for commercialization in Eastern and North Eastern India.

Region-wise selection of hardy Multi x bivoltine and bivoltine x bivoltine hybrids for increasing   cocoon and silk production.

6

PRE-3508: Studies on standardization of mass multiplication and field efficacy of Scymnus pallidicolli (Mulsant) for eco-friendly management of Tukra. (Apr., 14  to Mar., 16)

  • To study the intrinsic rate of increase of the native predator in ambient conditions (as suggested by referees while evaluating the project).
  • To standardize the mass multiplication technique of the native predator.
  • To determine the optimum number of native predators that are required to be released per unit area.
  • Life cycle of the predator Scymnus pallidicolli (Mulsant) under ambient conditions is 23- 25 days.
  • Recorded Multiplication rate of the pest (M. hirsutus) and its predator (Scymnus pallidicolli) was under laboratory conditions.
  • Released predators at field level

Mass multiplication of predator, Scymnus pallidicolli (Mulsant) and evaluate predatory efficiency of Scymnus pallidicolli (Mulsant) will be popularized against mealy bug (M. hirsutus) for effective control of mealy bug.

To suppress mulberry pests and saving of crop loss.

7.

PRE-3511: Studies  on predatory efficacy of coccinellid predator, Scymnus posticalis Sicard for  management  of white fly on mulberry.
(Apr., 14 to Mar., 16)

  • To evolve standardization of mass multiplication technique of the predator, scymnus posticalis sicard.

 

  • To evaluate predatory efficiency of scymnus posticalis sicard against whitefly, dialeuropora decempuncta on mulberry
  • Mass culture of mealy bug was done on potato sprouts and pumpkin. After release of 100,

200 &  300 mealy bug egg masses on pumpkins within a period of 30 days., it was multiplied 8565, 12438 & 15647 in numbers

  • Life cycle of the predator completed within 23-31 days.
  • Biological attributes of the predator on mealy bug revealed prolong life span and it  could not be multiplied in large scale.

Standardization of mass multiplication technique for the predator, scymnus posticalis sicard and its predatory efficiency against whitefly, Dialeuropora decempuncta on mulberry will be helpful in IPM system.

To suppress mulberry pests and saving of crop loss.

DURING THE YEAR:  2016-2017

1.

PIB-3479: Development of high yielding mulberry varieties using physiological growth parameters as markers for selection. (Oct., 2012 to Sept., 2016).

To develop mulberry varieties with superior quality and with 10% higher leaf yield over existing ruling variety.

1. Total of 1024 progenies, derived from six female and seven male parents were evaluated for growth traits and leaf yield   in five crop seasons during 2015-16.

2. The progenies recorded significant variation for physiological growth traits viz.,  total Chlorophyll content (11.1-30.4µg cm-2), Leaf area index (5.3-13.2), Leaf weight ratio (0.4-0.56), Leaf area duration (2.9-7.3 m2day-1), absolute growth rate (18.15-33.91 g day-1) and specific area (185.3- 372.13cm2g-1).

3. Based on annual leaf yield and physiological traits, twenty four superior progenies were shortlisted with leaf yield range from 2.104 to 2.561 kg/plant compared to check S-1635 (1.620 kg/plant).

4. Moreover, propagation traits viz., cutting survival (%), shoot and root lengths were observed to be at par/better than check S-1635.

1. Thorough evaluation of identified promising (~24) lines for further yield trials (PYT) before field utilization.

 

Utilizing the leads of the project a new project Code No. PIB 3610Preliminary evaluation of newly evolved mulberry genotypes for mulberry improve-ment” has already been initiated.

2.

AICEM-III: All India Coordinated Experimental Trail for Mulberry (AICEM)- Phase III, (A prog. of C.O., Bangalore) (Aug., 2011 to Dec., 2016).

To identify and authorize suitable mulberry variety for commercial use in different agro-climatic mulberry cultivation zone of India.

1. Mulberry genotype C-2038 exhibited highest average annual leaf yield production   (53.8 t /ha / yr in irrigated and 17.3 - 21.3 t/ha/yr in rainfed)  and excelled the check (S-1635) by 21% and 23% in irrigated and rainfed condition of  Eastern and North Eastern regions.

2. In hilly region of Kalimpong, Tr-23 appeared best performing genotype and showed 10-12 t/ha/yr ie., ~ 42% higher leaf yield over the check S-146.

 

The promising genotype will be popularized through extension programme.

Will enhance the production of mulberry leaves per unit area, farmers will be encouraged with the gain   through this technology.

3.

PIB-3515: Evaluation of new developed triploid mulberry varieties for productivity and quality. (Jun., 2014 to Mar., 2017).

Evaluation of newly developed triploid mulberry varieties under irrigated condition for better productivity and quality.

Identified 7 genotypes with significantly higher leaf yield 370 -445 g/crop (21.9 to 46.8 %) over the ruling variety, S-1635.
The selected genotypes were also found to be superior in their biochemical parameters and at par with check for physiological traits like moisture content and moisture retention capacity.

Identified genotypes with leaf yield may be evaluated thoroughly under FYT for commercial exploitation.

Identified promising genotypes need to be evaluated in large scale to identify one best one for commercial exploitation.

4

APS-3539: Characterization of mulberry growing soils for nutrient management in selected Seri-villages of Golaghat district of Assam. (April, 2015 to March, 2017) (Collaborative with NBSS&LUP, ICAR, Jorhat)

  1. To characterize and classify the mulberry growing soils.
  2. To prepare soil test based fertilizer recommendation chart for the mulberry growing soils.
  3. To popularize the soil test based fertilizer application among the farmers as per the fertility rating chart developed.

1. Recommended 0.1 % foliar sprays of boron along with recommended dose of fertilizer (RDF) for micronutrient Boron deficit mulberry growing soil.

2. Recommended NPK (150:50:50) per ha for medium fertility soil.

3. Recommended 25 % extra of RDF for low fertile soil and 15% less of RDF for high fertile soil.

1. Selection of 20 farmers (5 in each district), Jorhat, Golaghat, Dibugarh and Derang.

2. Demonstration and popularization of sericulture farmers.

Soil fertility status will be sustained. Besides, it will reflect in the production of mulberry leaves per unit area.

 

 

5

PPS-3504: Study on root rot disease of mulberry in the Gangetic plains of West Bengal and development of its control measures.  (April,  2014 to Mar., 2017)

  1. Survey and collection of root rot disease from the Gangetic plains of West Bengal.
  2. Isolation and identification of the organism causing root rot disease of mulberry.
  3. Recording of severity of root rot disease of mulberry in different locations of the Gangetic plains of West Bengal.
  4. Isolation of fungicide as control measure (In vitro) for plant root protection method including bio control agents and chemical management.

Survey revealed that root rot was predominant in the Gangetic plains of West Bengal. Root rot pathogen was identified as Fusarium solani from IARI, New Delhi and NFCCI, Pune.
0.20% SAAF (Carbendazim 12% + Mancozeb, 63%) application was found effective with 94.4% growth inhibition of F.solani under in vitro condition. Application of biocontrol agent Trichoderma viride was found effective with 66.22% growth inhibition of F.solani.
Management approach was popularized among farmers using pamphlets.

Application of 0.20% SAAF (Carbendazim 12% + Mancozeb, 63%) for the management of root rot in mulberry.

Timely management will help to reduce the damage caused by root rot disease; farmers will be benefitted through this technology.

6

CSS-2107: Forewarning of mulberry diseases of Eastern and North Eastern India. (April, 2012 to Mar., 2017)

  • To collect disease incidence and meteorological data from Eastern and NE India.
  • Recommendation of forewarning system package in different locations.
  • Development of long term and broad spectrum data base on disease severity.
  • Fine tuning of disease forecasting models at the end of XII th plan.
  • Fine tuning of existing disease calendar at the end of XII th plan.

Meteorological data and disease severity from different centers were collected and compiled.
Forewarning calendar and spray schedule developed for PMLD, BLS, MLS, PLS and LR.
Developed disease forecasting model for BLS (Bacterial leaf spot disease), PLS (Pseudocercospora leaf spot), PMLD (Powdery mildew), LR (Leaf rust) and MLS (Myrothecium leaf spot) in Murshidabad district, MLS for Malda district, LR and PMLD  for Kalimpong, PMLD for Aizawl, and Agartala, LR for Dimapur, PMLD  for Imphal, LR for Koraput and Rangpoo.

With changing climate disease incidence and severity is changing which needs to be monitored continuously.

Programme need to be continued to generate data on the changes in disease occurrence  due to climate change

7

AIB 3501: Development of multivoltine breeds of silkworm (Bombyx mori L.) with high shell percentage and high neatnes of silk filament. (July, 2013 to June, 2016)

To develop multivoltine silkworm breeds with better quality silk having higher shell content and high neatness of silk filament to suit the adverse climatic condition of West Bengal.

Seven multivoltine lines with high SR% (more than 17%) and high neatness (more than 90 points) have been developed as follows:
1. M.Con.1x MH1 (M1)-White
2. M.Con.4 x MH1 (M2) –Yellow
3. Nistari x MH1 (M3) –Yellow
4. M6DPE x MH1 (M6) –Yellow
5. MH1 x BHB (M7) –White
6. Gen3 x M.Con.4 (M4) –Yellow and
7. SK6x7 x M.Con.4 (M5) –Yellow
Besides, best two (Multi x Multi) and two (Multi x Bi) hybrids are also identified. These are
Multi x Multi Hybrid:
1. M7 x M.Con.4- SR%-18.60,  Neatness-84points & ERR(Wt)-  14.81
2. M1 x M9A - SR% - 18.37, Neatness-84points & ERR(Wt)-  16.47
Multi x Bi hybrid:
1. M2 x (B.Con.4 x 1)-  SR%-19.55, Neatness – 80 points & ERR (Wt)-  15.78
2.M1 x MC4(O)- SR%-19.67, Neatness-85points & ERR(Wt)-  15.97

Shortlisted breeds are being utilized in large scale trail through the implementation of new project entitled AIB-3616: On farm trail of the multivoltine silkworm breeds/hybrids developed for high shell percentage and neatness of silk (Sept., 2017 to Dec., 2020).

These breeds/ hybrids are helping to produce high quality silk having higher shell content and higher neatness of filament.

 

8

AIB 3480: Development of silkworm Bombyx mori L. breeds from a gene pool with higher genetic plasticity.  (Sept., 2012 to Aug., 2016)

Raising of poly hybrid gene pool having genetic plasticity in tolerance and in high cocoon shell weight and development of congenic breed from a selected lines for high cocoon shell weight and bivoltine breeds for horizontal tolerance

The following lines are developed:-
Bivoltine
Six way cross i.e., (B.Con.4 x CSR-2) x (Dun-21 x KPG-A) x (RSJ-14 x APS-45)-raised as converged  gene pool for high Shell weight and two lines were isolated as:

  • Plain (p) Larvae, Faint Constricted, White (c)  colour Cocoon
  • Marked (+p) Larvae, Faint Constricted, White (c) colour Cocoon

Multivoltine:
Six Way cross   i.e.,  (Nistari +p) xCambodge) x (M.Con.4 x PM) x (MH1 x Sarupat) – raised as  converged gene pool for horizontal tolerance (survival) and Three lines were isolated as:

  • Plain (p) Larvae, Yellow (C), Oval shape Cocoon
  • Plain (p) Larvae, White (c), Oval shape Cocoon
  • Plain (p) Larvae, Light Greenish(Gr) Oval shape Cocoon

Multivoltine and Bivoltine Congenic Line:
These lines have been used to develop 2 multivoltine congenic lines (RBL1) with high shell weight (>0.24g) and 2 bivoltine congenic lines (RBL1) with high survival (>90%).

Developed breeds are having higher genetic plasticity. But  development of these congenic lines are further continuing under new project  namely AIB-3619 as Phase-II.(July, 2017 to June, 2020).

9

ARP 3516: Studies on synbiotic (Combination of probiotic and pre biotic) induction for control of common disease of silkworm, Bombyx mori L.  (Oct., 2014 to Sept., 2016)

Application of synbiotics (combination of probiotics and prebiotics) for eco-friendly silkworm disease management.
Strengthen the immunity of silkworm to resist the microbial pathogenic attack.
To promote good cocoon yield.

The synbiotics tested in the present study were not showing profound influence on the characteristics tested in silkworm. The probiotic microbes tested in the present study were proved to be successful in humans but it could not produce the same effect in silkworm system as the physiological, biochemical, immunological aspects and interaction of host native microbiota are different among both the systems. The strain specificity of probiotic microbes also plays a significant role in producing the beneficial effect on the host.

Tested probiotic strains are not found to be working against silkworm.

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10

AIB 3466: Development of region specific bivoltine breeds suitable for highly fluctuating and seasonally variable climatic conditions of Eastern and North-Eastern India.  (Aug.,  2011 to Dec., 2016)

Region specific bivoltine breeds suitable for highly fluctuating and seasonally variable climatic conditions of Eastern and North-Eastern India to be developed.

Five new oval (BHP-1, BHP-2, BHP-3, BHP-4 and BHP-5), and five new dumbbell breeds (BHP-6, BHP-7, BHP-8, BHP-9 and BHP-10) with better genetic plasticity are developed.

New breeds developed under the project needs to be subjected for hybrid studies.

Newly developed breeds (BHP-1 to BHP-10) with better genetic plasticity are utilizing for further study under the new project AIB3617 as Phase-II (April, 2017 to March., 2020)

11

PPF 3532: Assessment, Development and Management of area under mulberry in major sericulture districts of West Bengal. (Feb., 2015 to Jan., 2017)

To estimate the current spatial extent of mulberry cultivation in selected blocks of 4 major Mulberry growing districts of West Bengal using Remote Sensing, Geographic Information System and Global Positioning Systems.
Make an attempt to estimate leaf protein and moisture contents using hyperspectral data with limited laboratory based analysis.
To develop block specific MIS which can be integrated with SILKS portal for dynamic visualization.

Using geo-spatial technique, standing mulberry area were assessed in 4 Districts viz., Malda, Birbhum, Nadia and Murshidabad of WB  in different season and at different growth level.
Co-relation between hyper-spectral radiometry values and optimized soil adjusted vegetation Index (OSAVI) against biochemical parameters was established for first time for mulberry crop condition assessment.
Farmers wise MIS details viz., area of garden, variety of mulberry etc mapped  in one of the village of each District for the first time.

The study shows the potential of geospatial technology for acreage estimation of mulberry crop at block level within acceptable error limit. Hyperspectral data could effectively be used for estimation of leaf moisture and mulberry leaf protein content. This will open new area of further research in studying various biochemical properties with hyperspectral data. MIS developed for the selected four villages shows the potential of using open source GIS platform for farm level planning and design making through a geoportal. The MIS may be linked to institutional websites to have wider use by different stakeholders of the sericulture industry in the state.

Timely and accurate estimation of mulberry acreage will play critical role for the planners, decision makers and other stakeholders.

DURING THE YEAR:  2017-2018

1

PPE-3517: Population Interaction of pest and natural enemies in mulberry ecosystem. (Aug., 2014 to July, 2017) (Collaborative with NBAIR, Bangalore)

To study the population interactions of host plant, major mulberry pests and their natural enemies and correlation with abiotic factors. 
Studies on the biological attributes of the parasitoids.

The life stages of the pests were collected from the field and screened in the laboratory for the emergence of parasitoids & predators.

  1. Studied the effect of the major mulberry pests and their control by natural enemy complex.
  2. Made a list of alternate hosts for pests and their natural enemies.
  3. Migration pattern of natural enemy complex will be explored

Will explore the possibility to inhibit the growth of pest and increase the population of natural enemies.

2

AIB 3514: Development of multivoltine based congenic /NIL breed of silkworm (Bombyx mori L.) through introgression of “Id” gene and its uses. (June, 2014  to  May, 2017)

Development of homozygous Id gene carrier Multivoltine based Congenic /NIl through introgression.

Five Id character carrier breeds have been developed with 100% hatching without acid treatment.
1.M.Con.4Id (Pseudo pigmented Diapause Inhibitor Yellow Oval  shaped cocoon) similar to M.Con.4
2.M.Con.4 Id(Non pseudo Diapause Inhibitor Yellow Oval  shaped cocoon) similar to M.Con.4
3.M.Con.4 Id (Pseudo Pigmented Butter Colour Cocoon
4. B.Con.4 Id Pseudo Pigmented Butter Colour Cocoon.
5.BHB Id Pseudo Pigmented White Colour Oval & Dumbbell

It will be undertaken for large scale trial as Bi x MultiId hybrid, for which acid treatment is not required

Bi x MultiId hybrid helps to utilize both female & male moths can be utilized during the grainage.
It ensures the hatching of dfls of these hybrids without any acid treatment.

3

AIB 3547: Development of high temperature and high humidity tolerant bivoltine breeds of silkworm (Bombyx mori L.) (July, 2015 to June, 2017)

To develop thermo-tolerant Bivoltine silkworm breeds with genetic plasticity to buffer against the adverse climatic conditions of West Bengal.

Ten new silkworm breeds:
Two hybrids viz., HTH-3 x HTH-6 and HTH-4 x HTH-9 have been identified as tolerant to high temperature (35º C) and high humidity (>90%).

A new project will be taken for on farm trial with Identified hybrids.

In tropical areas of sericulturist are facing unfavorable conditions of environment that affects directly to the silk production.
Rearing of thermo-tolerant breeds will be one of the most effective methods to increase silk productivity.

4

AIB 3545: Authorization Trial of Silkworm hybrids in Eastern and North Eastern India. (Aug., 2015 to March, 2018). [Collab. With NSSO, Bangalore and CSTRI, Bangalore]

To evaluate the newly developed, Multi x Bi and Bi x Bi hybrids at the farmers level in Eastern & North Eastern Zone.
To     popularize        the       newly developed hybrids in Eastern & NE Zone.
To nalyse the data of the tested hybrids.
To identify the suitable hybrids for the Eastern& NE Zone.
Recommendation of the hybrids for the Eastern and NE zone for commercial exploitation.

Under the authorization trial,
One new Bi x Bi hybrid- B.Con.1 x B.Con.4 and one new Multi x Bi hybrid- M6DPC x (SK6 x SK7) were tested during 2014 to 2017 in West Bengal, Jharkhand, Odisha and North-East states.
The avg. yield of 1,69,100 dfls was 50.25 kg/100 dfls in B.Con.1 x B.Con.4 as compared to 94,800 dfls of SK6 x SK7 (46.80 kg/100 dfls).
The avg. yield of 2,10,150 dfls was 47.96 kg/100 dfls in M6DPC x (SK6 x SK7) as compared to 1,03,300 dfls of N x (SK6 x SK7) (43.15 kg/100 dfls).

Utilizing B.Con.1 x B.Con.4 as Bi x Bi hybrid in place of SK6 x SK7 and M6DPC x (SK6 x SK7) as Multi x Bi hybrid in place of N x (SK6 x SK7).

This authorized silkworm hybrids will definitely enhance the production of silk as well as quality.

5

AIT 3557: Conduct multi-locational trial on transgenic Bm NPV resistant silkworm strains to establish their efficacy and generate data for their regulatory approval. (January, 2016 to August, 2017) (Collaborative with APSSRDI, Hindupur, Andhra Pradesh)

To establish the efficacy of Bm NPV resistant silkworm strains and generate data for their regulatory approval

Non-significant difference is observed upto 5th cycle of comparative rearing of Transgenic [N(T) X (SK6 x SK7)] and Non-Transgenic [N x (SK6 x SK7)].

The hybrid N x (SK6 x SK7) can be used as equally resistant of N(T) X (SK6 x SK7).

 

Resistant against the diseases helps to produce more amount of cocoon and silk, helps to get more income for the producers.

6

PIN 3587: Improvement of leaf quality and productivity through external application of seaweed extracts in mulberry (Morus alba L.) (Oct., 2016 to Sept., 2017).

To determine the effect of application of seaweed extracts on growth and biomass in mulberry leaves.
To standardize the dosage and time interval for the application of seaweed extract.
To determine the effect of application of seaweed extracts on foliar diseases of mulberry.
To perform bioassay on silkworm larvae fed with seaweed extract treated mulberry leaves.

Application of seaweed extract (Ascophyllum nodosum @ 0.5 ml/L) sprayed at  21 and 28 days after pruning, at 60th day increased leaf yield (11 %) compared to control S-1635 (328.33 g/plant) (average of 30 plants).

To meet increasing demand of organic inputs, among many viable options, one option is the use of seaweed extracts as a source of nutrients and hormones. Seaweed extracts application is expected to minimize the hazardous effects of synthetic chemicals and nutrients in the ecosystem.
New project will be taken up for large scale trials to validate the result

External application of seaweed extract is expected to improve the quality and quantity of mulberry leaves organically which in turn would have a direct bearing on the yield and the quality of cocoon

7

PIB 3481: Evaluation of mulberry varieties suitable for low in put soils. (Jan., 2013 to Dec., 2017)

To evaluate newly evolved promising mulberry varieties suitable for low-input soils.

Out of 7 test genotypes, C-9 recorded significantly higher leaf yield compared to check S-1635 i.e 13.07 % & 26.6% higher under full RDF & Half RDF, respectively for irrigated condition. Similarly, 27.85% higher in case of Rainfed condition.

The promising genotype will be popularized through extension programme.

Will enhance the production of mulberry leaves per unit area, farmers will be encouraged with the gain through this technology.

8

PIB 3521: Assessment of promising powdery mildew resistance lines for perspective commercial use. (Jan., 2015 to Dec., 2017)

Evaluation of powdery mildew resistant promising lines for foliage biomass and associated ancillary traits.
Evaluation of silkworm rearing efficiency of the selected line(s) through bioassay.
Development of segregating progeny using sib-mating from promising F1 lines.
Assessment of powdery mildew responsiveness of developed progeny using identified markers for MAS based utilization.

Two promising lines exhibited ~ 17-29% more leaf biomass than S-1635 (44-45 t/ha/y).

One of them (C-1360) selected for AICEM IV phase trial by MVAC (2018-2023)

Reduce the crop loss due to PM disease especially in favorable season of silkworm rearing, will enhance the production of mulberry leaves.

9

PRE 3533: Incidence of whitefly in mulberry germplasm accessions. (Mar., 2015 to Feb., 2018)

To observe the incidence of whitefly in the mulberry germplasm for selection of putative sources of resistant and susceptible variety for whitefly infestation.
To evaluate the selected germplasm for white fly resistance using physiological and biochemical parameters.

Discriminated mulberry accesssions of CSR&TI, Berhampore as resistant and susceptible based on whitefly incidence.

The putative resistant and moderately    resistant mulberry sources would be a best source of breeding material with whitefly resistance.

Use of identified accession reduces the crop loss from the whitefly and also helps in mulberry breeding for whitefly resistance. Ultimately it may helps to increase in mulberry production.

10

PPS 3559: Testing of carbon capturing potential in mulberry in different location. (April, 2015 to March, 2018)

To assess carbon capturing efficiency of Mulberry growing under varying eco-geographical condition.
To examine the validity of the technology for spraying of cationic micronutrients in mulberry garden at farmers level.

Moderate tillage with grass cover registered carbon capturing of ~ 9% improvement (632 kg/ha/crop) as compared to intensive tillage without grass cover (576 kg /ha/crop)

Standardized    farming protocol in terms of maximizing   carbon sequestration in mulberry cultivation will be popularized among the sericulture farmers to meet of the current global needs.

It is would be useful in enhancing leaf production.

11

PPA 3499: Evaluation of field level performance of Vishala mulberry variety in different locations under irrigated conditions in West Bengal. (April, 2013 to March, 2018)

To find out the potentiality of Vishala mulberry variety under irrigated conditions in West Bengal.
Evaluation of field performance of Vishala mulberry variety in different locations under irrigated conditions in West Bengal.

Potentiality of Vishala mulberry variety in WB (irrigated) two years harvest data of 23 experimental plots [500 plants/ plot] of 4 districts of WB revealed yield 38.95 t/ha against 35.72 t/ha in control(S-1635).

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12

BPI (PS) 010: Identification of bio-chemical markers for thermo-tolerance in silkworm (Bombyx mori L.)  (Oct., 2016 to Sept., 2017).

Identification of biochemical markers associated with ROS defense in bivoltine silkworm under thermal stress.
Establishment of correlation between ROS defense associated enzymes and yield parameters.

Super oxide dismutase (SOD) activity and H2O2 titres showed highly significant correlation (P<0.05) with survival and pupation of the silkworm (BCon4 > BCon1 > BHR3) exposed to high temperature (35°C with 75±5% RH)

A  new project is needed to establish regarding:
Better performing breeds (BCon4, BCon1 and BHR3) need to be studied further for their consistent performance under specific intermittent thermal stress regime beyond 25°C to 35°C.
To elucidate the protective role of SOD as an additional marker of thermo- tolerance in silkworm.

Testing the usefulness of   ROS stabilization associated biochemical parameter(s) as prospective rapid / stable screening tools to elect thermal stress tolerant silkworm strains for breeding utilization.

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B-JRH (P) 040: Studies on mulberry germplasm in agroclimatic condition in North Eastern States. (April, 2015 to August, 2017).

To establish mulberry field gene bank with the collections from north-eastern region.
To identify a promising mulberry accession for commercial utilization.

Among the 23 accessions studied, MI -0349 (Garbandha) is recommended for further multiplication.

The mulberry accessions are being utilized in the mulberry breeding program.

Maintaining the mulberry accessions for further breeding programme. It would be a continuous process.