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Silkworm Pathology SCIENTISTS: 1)
Dr. Satadal
Chakraborty, Scientist-B (Sectional Head) MANDATE: 1.
To evolve integrated silkworm disease management
strategies for the prevention/ control of silkworm diseases 2.
To act as a nodal centre for co-ordinating silkworm
disease survey and surveillance programme in the eastern and north-eastern
parts of 3.
To extend R&D support to the sericulturists in
the eastern and north-eastern parts of ACHIEVEMENTS: Ø LABEX a
general silkworm bed-disinfectant, developed by this section, has been
patented (Patent No. 200199) and commercialized. Ø A room
disinfectant, 5% Bleaching powder solution has been developed for the
prevention of various silkworm diseases Ø Developed
a new general silkworm bed-disinfectant, SERICILLIN specifically for
control of muscardine disease. Ø Gattine
disease is controlled by using 1% chlorine (bleaching powder solution) as
room disinfectant and feeding of 700ppm Streptomycin sprayed leaves including
dusting of Labex. Ø Identified
two botanicals for control of muscardine and grasserie diseases Ø Elucidated the structure and nature of
haemocytes of Bombyx mori L. and their role in the cellular defensive
response. Ø Identified
disease tolerant silkworm races from the germplasm based on haemocyte
immunity study Ø Developed
a simple agglutination test for early detection of BmNPV infection in B.
mori L. Ø An Agar
Gel Double Diffusion Assay has been standardized for distinguishing the
various serotypes of Bm NPV Ø Identified
hotspots for various disease occurrences in RESOURCES: Ø
Autoclave Ø
Centrifuge(REMI) Ø
Cyclomixer Ø
CO2 Incubator Ø
Leitz Diaplan compound
microscope, Ø
Laminar Flow Ø
Water Purification System
(Sartorius) Ø
Cell Sonicator ONGOING PROJECTS / PILOT STUDY: ARP3351: Studies on the morphology, life cycle and pathogenicity
of some microsporidia infecting silkworm, Bombyx mori L. and their
identification Objectives: a.
To study the biology of microsporidia b.
To identify the virulent microsporidia based on
their pathogenicity to Bombyx mori L. c.
To investigate the impact of microsporidian
infection on growth and development of Bombyx mori L. d.
To identify the molecular markers
for the identification of virulent and non-virulent microsporidia. PILOT
STUDY: Immunization of silkworm, Bombyx mori L. against bacterial disesase
Objectives: a.
Immunization of disease tolerant silkworm breeds
identified so far. b.
The resultant protein rich compound / heat killed
bacteria /other preferably low cost chemicals/botanicals suitable for
immunization may be patented for the benefit of Sericulture industry. CONCLUDED
PROJECTS: ARP3285:
Studies on the pathogens, pathogenesis and control of gattine in
silkworm Bombyx mori L Outcome:
It was found that Gattine disease is caused by an ultra virus and a
bacterium (Staphylococcus vitulinus). Gattine disease is controlled by
using 1% chlorine (bleaching powder solution) as room disinfectant and
feeding of 700ppm Streptomycin including dusting of Labex as per
recommendation, without affecting the economic characters of the cocoons. ARP3286: Studies on the prevention
and control of Nuclear Polyhedrosis and White Muscardine diseases of silkworm
using botanicals Outcome:
Identified an eco-friendly, effective and economically viable two botanicals
for the prevention/ control of Grasserie and White Muscardine
diseases of silkworm, Bombyx mori L. Spray of 5% aqueous crude extract of PE-02 after every moult
(30-45 m before resumption of feed) and 4th day of 5th instar is found to be very effective against
White Muscardine Spray of 7% aqueous crude extracts of PE-10 is
used as a preventive/ control measure against
Grasserie disease by per oral administration through
mulberry leaves after each moult (30-45 m before resumption of feed) and on 4th day of
5th instar ARP004: Studies on immunity and
immunodiagnosis of silkworm diseases Outcome:
A simple agglutination test has been developed for detection of BmNPV
infection. An agar gel double diffusion assay has been standardized for
distinguishing the various serotypes of BmNPV ARP008: Haemocyte immunity study of silkworm, Bombyx
mori L. Outcome:
On the basis of cellular defense response to bacterial/ particulate
virus infection, five multivoltine [Nistari, Debra, Nistari (DN)P, M6DP &
M12(W)] and four bivoltine (SK3, SK6, BHR1 and BHR2) were identified as
relatively tolerant from the germplasm ARP002: Physiotherapeutic and
chemotherapeutic studies towards control of silkworm diseases Outcome: Recommended hot HCl treatment (1.05 sp.
gravity at 46ºC for 30 m) for controlling pebrine at primary infection and
Albendazole (0.5%) as feed to control pebrine at secondary infection. Nystatin
(0.4%) was identified and recommended to control White Muscardine disease ARP003:
Studies on the improvement of disinfection
Labex, a
silkworm bed-disinfectant and 5% Bleaching powder solution, an
effective room disinfectant were formulated and recommended for the
prevention of various silkworm diseases ARP005: Survey and surveillance of silkworm
diseases and its management in
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